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(Panels a, b) Single molecule fluorescent intensity time-traces of immobilized packaged Cy5-Cy5.5 labeled 5kbp DNA as seen in the donor and acceptor channels recorded simultaneously by two SPAD detectors. (c,d) Corresponding FRET efficiencies from the double dye labeled packaged 5kbp DNAs. e) Single molecule FRET efficiency of packaged Cy5-Cy5.5 labeled 5 kbp DNA. Data in this bottom panel was compiled from about 150 individual time-traces.
