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(Panels a, b) Single molecule fluorescent intensity time-traces of immobilized packaged Cy5-Cy5.5 labeled λ-DNA as seen in the donor and acceptor channels recorded simultaneously by two SPAD detectors. (c,d) Corresponding FRET efficiencies from the double dye labeled packaged λ DNAs. e) Single molecule FRET efficiency of packaged Cy5-Cy5.5 labeled 50 kbp λ DNA. Data in this bottom panel was compiled from about 120 individual time-traces.
