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. Author manuscript; available in PMC: 2011 Feb 1.
Published in final edited form as: Mol Carcinog. 2010 Feb;49(2):152–156. doi: 10.1002/mc.20584

Figure 3. Inactivation of E2f2 can positively or negatively effect gene expression.

Figure 3

(A) cDNA was made from epidermal keratinocytes isolated directly from mice with the indicated genotypes and used in real-time quantitative RT-PCR assays for the indicated genes. Results are the average of three independent assays performed in duplicate using RNA isolated from 3 different mice of each genotype. Statistically significant differences in expression between wild type and E2f2−/− genotypes were found for Rprm and Tsc22d3 and between K5.Myc, E2f2+/+, and K5.Myc, E2f2−/− genotypes for Serpine1 and Tsc22d3 (paired t-test, p < 0.001). (B) Western blot analysis of epidermal lysates from mice with the indicated genotypes was performed using antibodies specific for cyclin E (recognizes both the cyclin E1 and E2 proteins) and GAPDH as a loading control.