Fig. 3.

Firefly and renilla luciferase reporter gene activities in Brugia malayi embryos transfected with putative operon constructs. Constructs were prepared in the bicistronic vector and transfected into B. malayi embryos as described in Materials and methods. A) Constructs containing the promoter and intergenic regions from the putative 14933 operon. B) Constructs containing the promoter and intergenic regions from the putative 14406 operon. In each panel, paired columns represent firefly (F) and renilla (R) luciferase activities obtained from the same construct. Columns represent the mean, and error bars the S.D. of data obtained from six independent transfections. The tables below each graph indicate whether the promoter and/or intergenic domain was present or absent in each construct. Activities are expressed in as relative light units (RLU) (raw light units minus background) per milligram of total protein in the extract.