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. Author manuscript; available in PMC: 2011 Jan 1.
Published in final edited form as: Int J Parasitol. 2009 Jul 23;40(1):63. doi: 10.1016/j.ijpara.2009.07.001

Fig. 5.

Fig. 5

Analysis of the role of putative U-rich (Ur) domains in expression of the downstream renilla reporter gene in the 14933 operon. A) Sequence of the 14933 intergenic region. The putative Ur elements are indicated by bold type. Italic type indicates the sequence corresponding to the 5’ end of the downstream open reading frame (ORF) of the Brugia malayi pre-mRNA splicing protein prp5 homologue. The putative poly A addition signal for the upstream gene is indicated by underlining. The arrow indicates the addition site for spliced leader 1 (SL1) on the native the B. malayi pre-mRNA splicing protein prp5 homologue mRNA, determined by DNA sequence analysis of the amplicon shown in Fig. 4. B) Firefly and renilla luciferase activity in embryos transfected with bicistronic constructs containing mutations in the putative Ur domains. Ur domains are numbered by their relationship to the putative upstream poly A addition site, with Ur#1 representing the element closest to the poly A addition site. Columns represent the mean, and error bars the S.D. of six independent transfections. Activities are expressed in as relative light units (RLU) (raw light units minus background) per milligram of total protein in the extract.