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. Author manuscript; available in PMC: 2011 Jan 1.
Published in final edited form as: Atherosclerosis. 2009 Jul 23;208(1):104. doi: 10.1016/j.atherosclerosis.2009.07.029

Fig. 2.

Fig. 2

Effects of EFV and MnTBAP on the expression of junctional molecules in HCAECs. HCAECs were treated with serial concentrations of EFV (1, 5 and 10 µg/ml) for 24 hrs. (A) The mRNA levels of ZO-1, claudin-1, occludin, JAM-1 and VE cadherin were analyzed by real time PCR, and values were normalized to β-actin. n = 3. *P < 0.05. (B) The protein levels of ZO-1, occludin, claudin-1 and JAM-1 were analyzed by western blot analysis. Relative density of the expressed proteins was normalized to the corresponding β-actin band as the fold of the control. (C) Histogram of flow cytometry analysis showing protein levels of ZO-1, claudin-1, occludin and JAM-1 under control and EFV treated conditions. HCAECs were treated with 5 µg/ml EFV for 24 hrs.