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. Author manuscript; available in PMC: 2010 Jan 29.
Published in final edited form as: Exp Neurol. 2005 Mar;192(1):11. doi: 10.1016/j.expneurol.2004.05.038

Fig. 5. RC-1 up-regulation by reactive astrocytes and ependymal cells.

Fig. 5

(A) RC-1, a marker for radial glia, is up-regulated by cells surrounding EB lesions. In (A–F), the inset shows the entire region of the VLF and the inner box illustrates the areas shown at higher magnification. (B and C) GFAP double labeling reveals that RC-1+ cells are GFAP+-reactive astrocytes. (D– F) RC-1 up-regulation by reactive astrocytes around the lesion is maintained at 28 dpi. (G) RC-1 is also up-regulated by ependymal cells surrounding the central canal at 7 dpi. (H) GFAP is expressed by subependymal astrocytes, but not ependymal cells. (I) Twenty-four-hour BrdU pulse labeling at 7 dpi reveals that many ependymal cells are dividing at this time. (J) Merged image of (G– I). (K) At 28 dpi, ependymal cells no longer express RC-1. (L) GFAP expression by subependymal astrocytes at 28 dpi. (M) Twenty-four-hour BrdU pulse labeling at 28 dpi shows that ependymal cells are no longer dividing at this time. (N) Merged image (K– M). Scale bar = 30 µm in (A– F); 25 µm in (G–N).