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CHO cells transiently transfected with empty vector or the indicated Rspo2 construct were fixed and stained under non-permeabilization conditions to visualize Rspo2 proteins on the cell surface or in the extracellular space. Rspo2 proteins were detected with V5 antibody, while cell boundaries were indicated by phalloidin staining of polymerized actin and DAPI stained nuclei.
