TABLE II. Identification of specific associated proteins of Rad53 under different conditions.
Detailed data can be found in supplemental Table II.
| Protein | Detected in | Average ratio (MMS/Control) |
No. of peptides quantitated |
SD | |
|---|---|---|---|---|---|
| CTL | MMS | ||||
| Rad 53 (YPL153C) | X a | X | 3 | 7b | 0.4 |
| Kap95 (YLR347C) | X | X | 2.2 | 12 | 0.5 |
| Srp1 (YNL189W) | X | X | 1.4 | 11 | 0.3 |
| Nup2 (YLR335W) | X | X | 6 | 2 | NAc |
| Asf1 (YJL115W) | X | X | 2.5 | 2 | NAc |
| Yta7 (YGR270W) | X | X | 2.6 | 9 | 1.1 |
| HTA1/HTA2 (YDR225W/YBL003C) | X | X | >10 | 2 | NAc |
| HTB1/HTB2 (YDR224C/YBL002W) | X | X | 5 | 6 | 1.7 |
| HHF1/HHF2 (YBR009C/YNL030W) | – | X | NAd | 2 | NAc |
| Rad9 (YDR217C) | – | X | NAd | 2 | NAc |
a
X indicates the presence of the protein in the indicated sample.
b
Rad53 hyperphosphorylation led to the stoichiometric depletion of unphosphorylated Rad53 peptides, so we only considered peptides located in the TAP-tag region.
c
NA, not applicable when two or fewer peptides are quantitated.
d
NA, not applicable when the corresponding peptide is not identified in the control sample.