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. 2010 Jan 8;3:22. doi: 10.3389/neuro.04.022.2009

Figure 4.

Figure 4

Activity-dependent expression of Filamin A is aberrant after spaced training in the dFMR1 mutant. (A) Western blot analysis of Cheerio level before (−) or after (+) spaced training in wild-type flies (WT) or the dFMR1 (FmrB55) mutant. All lanes were loaded with 50-ug total protein. Anti-actin (lowest band for each lane) was used as loading control. All heads were collected similarly and processed in parallel. Protein mass markers are in kD. (B) Quantification of western blot analysis for the smaller isoform of Cheerio (105 kD). In wild-type (WT) flies, Cheerio short-form levels are similar before (−) and after (+) spaced training. In the dFMR1 mutant (FmrB55), however, the Cheerio short-form is significantly reduced. (C) Western blot analysis of Cheerio before (−) or after (+) massed training in wild-type flies (WT) or the dFMR1 (FmrB55) mutant. All lanes were loaded with 50-ug total protein. Anti-actin (lowest band for each lane) was used as loading control. All heads were collected similarly and processed in parallel. Protein mass markers are in kD. (D) Quantification of western blot analysis for the smaller isoform of Cheerio (105 kD). The Cheerio short-form levels are similar before (−) massed training in both wild-type (WT) and dFMR1 mutant (FmrB55) flies. After massed training, Cheerio short-term is significantly reduced in both wild-type (WT) and dFMR1 mutant (FmrB55) flies.