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. 2009 Nov 17;102(1):188–195. doi: 10.1038/sj.bjc.6605440

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Copyright © 2010 Cancer Research UK

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Secretion and regulation of SPARC protein expression in human pancreatic stellate cells (PSCs). (A) Detection of SPARC protein in conditioned medium. Conditioned medium of PSCs, PANC-1, and COLO-357 was collected for 48 h and subjected to immunoblot analysis after pull down of heparin-binding proteins by heparin sepharose. Cell lysates of PSCs were used as positive control (control), medium alone as negative control (medium). (B) Regulation of endogenous SPARC expression in PSCs. PSCs were incubated (treatment) with conditioned medium of PSCs, COLO-357, or PANC-1 cells for 48 h. SPARC immunoblot analysis of total PSC cell lysates after incubation is shown in the upper panel, relative SPARC protein expression after densitometric analysis in the lower panel. The results in the lower panel are mean SPARC levels (±s.d.) in relation to PSC (100%) of three separate experiments. ^*P<0.05 compared with control (PSC).