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. 2009 Nov 10;102(1):97–103. doi: 10.1038/sj.bjc.6605418

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Copyright © 2010 Cancer Research UK

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Combination of XAC 1396-11 and vinorelbine synergistically induces apoptosis of NSCLC cells. (A) H460 cells were treated for 72 h with vinorelbine, XAC 1396-11 or the combination±pan-caspase inhibitor (caspase In.), at the indicated concentrations below. Lysates were then prepared for immunoblot analysis using antibodies recognising PARP (full-length and cleaved ‘c’ PARP), XIAP, cIAP1, cIAP2, Survivin and GAPDH. Low concentrations of the single agents failed to induce PARP cleavage, whereas combination treatment provoked PARP cleavage (arrow). V1=vinorelbine 2.5 nM; X1=XAC 1396-11 5 μM; Caspase In.=50 μM; V2=vinorelbine 10 nM; X2=XAC 1396-11 10 μM; CTL=control (B). H460 cells were treated for 36 h with the first agent followed by removal of the supernatant and treatment for 36 h with the second agent, covering the concentration–effect curve (V=vinorelbine; X=XAC 1396-11). The plates were fixed and growth inhibition was determined by SRB. The CI results at the IC₂₅, IC₅₀ and IC₇₅ are shown. All experiments were repeated in triplicate, error bars show s.e.m.