Skip to main content
. 2009 Jun;124(3-4):228–238. doi: 10.1159/000218128

Fig. 3.

Fig. 3.

Neocentromere-containing chromosomes are distinguished from oat chromosomes. a DAPI (blue) staining and FISH using an OPIE probe (green) identifies stable neocentromere chromosomes in a metaphase spread, whereas a CentC FISH probe (red) does not stain. b Full addition line chromosomes are larger than their neocentromere counterparts and their native centromeres are identified by CentC staining. c FISH with a CentA probe (green) stains centromeres, pericentromeres, and speckles chromosome arms of maize B73 chromosomes. d The same CentA probe stains oat centromeres/pericentromeres, and stains the maize ch3S neocentromere chromosome asymmetrically (circled in red). e The unstable neocentromere chromosome CTo1_3 (red arrowhead) is a fraction of the stable size (a), and is symmetrical around a primary constriction.