Fig. 4.

Identification of the oat CENH3 protein. a A CLUSTAL [Larkin et al., 2007] alignment using the N-termini of the rice, maize, and oat forms of CENH3 proteins. b The underlined sequence was used to generate a peptide antibody that detects oat but not maize CENH3 in mixed cell immunolocalization-FISH experiments. Oat cells are larger and stain for CENH3 (red), whereas maize cells are smaller and stain for CentC (green) but not oat CENH3. c Oat pachytene and d metaphase I immunolocalizations using the oat CENH3 antibody (red or green), stain oat centromeres.