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. Author manuscript; available in PMC: 2010 Jan 29.
Published in final edited form as: Chem Biol. 2008 Apr;15(4):393. doi: 10.1016/j.chembiol.2008.02.017

Figure 2. Creation of OleD variants improved toward novobiocic acid (1).

Figure 2

(A) Specific activities of WT and mutant OleDs with novobiocic acid (1) and UPD-Glc (5) as acceptor/donor, respectively. (B) The crude cell extract glucosylation activities of randomly selected colonies from saturation mutagenesis libraries P67X, I112X, and A242X, with 1 as acceptor. Activities are illustrated in descending order and arrows designate clones that were selected for in-depth characterization.