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. Author manuscript; available in PMC: 2010 Jan 29.
Published in final edited form as: Chem Biol. 2008 Apr;15(4):393. doi: 10.1016/j.chembiol.2008.02.017

Figure 4. Probing the donor specificity of WT OleD and mutant prodigy.

Figure 4

(A) The set of UDP-sugar donors used to probe specificity. Dashed-boxed donors were detectable substrates for both WT and mutant P67T/I112T/A242V OleD, while solid boxed donors were substrates only for P67T/I112T/A242V. (B) Successful conversion rates (%) after 3 h with WT or P67T/I112T/A242V OleD using 50 μM acceptor 1 and 250 μM UDP-sugar donors (the reactions containing 28 were incubated for 18 h). (C) Improvement of donor promiscuity with increasing proficiency of OleD variants.