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. 2010 Feb 1;5(2):e8979. doi: 10.1371/journal.pone.0008979

Figure 3. Validation of CSNK1E as an essential gene in additional β-catenin-activated breast cancer cells.

Figure 3

(A) Immunoblot analysis of active (upper panel) and total (middle panel) β-catenin levels in five additional breast cancer cell lines, BT474, BT549, DU4475, HS578T, and SKBR3. (B) Immunoblot analysis of cytoplasmic (left) or nuclear (right) β-catenin levels. Immunoblots for cytoplasmic GAPDH and nuclear lamin were performed to verify fractionation. Effects of (C) CTNNB1 or (D) CSNK1E suppression on proliferation. Graph shows mean ± SD of a representative experiment performed in triplicate. (E) Immunoblot analysis of CK1ε levels after RNAi-induced suppression.