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. Author manuscript; available in PMC: 2011 Feb 5.
Published in final edited form as: Virology. 2009 Dec 2;397(1):176. doi: 10.1016/j.virol.2009.11.008

Figure 1. A35 inhibits the amount of antigen presentation-induced IL-2 and NO.

Figure 1

Freshly isolated PEC were isolated from a rat, infected with VV (MOI=2) for 5 hours, pulsed for 30 minutes with 50 nM GPMBP, and then incubated with the cognate CD4+ RsL.11 T cell line. Uninfected PEC served as a control. At 24- to 48-hpi, supernatants (50 ul) were collected and assayed for either IL-2 using the IL-2 dependent cell line CTLL (A) or for NO using Greiss reagent (B). For the CTLL bioassay, media only was used to define the background control level and known IL-2 containing supernatants were used as positive control. IL-2 production was determined as the mean OD value of the experimental group minus the background control level. * p < 0.05

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