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. Author manuscript; available in PMC: 2011 Feb 5.
Published in final edited form as: Virology. 2009 Dec 2;397(1):176. doi: 10.1016/j.virol.2009.11.008

Figure 2. A35 globally reduces antigen presentation-induced cytokine synthesis by APC and T cells.

Figure 2

PEC were harvested from Lewis rats, infected with WR or A35Δ virus (MOI=2) for 5 h, pulsed with 50 nM GPMBP, and then co-cultured with the cognate CD4+ RsL.11 T cell line. Supernatants (50 ul) were collected at 24-h post incubation and assayed for the production of cytokines using the LincoPlex 24 Rat Cytokine/Chemokine Luminex Bead Immunoassay Kit. IL-2 and IFNa values were divided by 20 and 100 respectively to fit to scale. IL-6, IL-18, and MCP-1 were not significantly reduced by the presence of the A35 gene in WR, all other differences between WR and A35Δ were p < 0.05.

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