Figure 3. A35 does not directly affect T lymphocytes.

(A) RsL.11 T cells were infected with WR or A35Δ virus (MOI= 4) for 4 h and then incubated with decreasing numbers of Ag pulsed PEC. After 24–48 h, supernatants (50 ul) were collected and assayed for IL-2 production using the CTLL IL-2 bioassay. (B) RsL.11 T lymphocytes were infected for 3–4 h and then stimulated with PEC/50 nM GPMBP, 25 ug/ml Con A, or 100 nM PMA/2 uM ionomycin. Supernatants (50 ul) were collected at 20- and 40-h and assayed for IL-2. (C) RsL.11 T cells were infected with VV for 10 h, and growth metabolism was measured by reduction of 10 ul MTS/PMS (D) CTLL cells were infected with VV (MOI=5) for 4 h and then placed in media with varying amounts of an IL-2-containing supernatant. Proliferation was measured by adding 10 ul MTS/PMS.