Figure 4. A35Δ mutant virus is as infectious as WR and replicates equally.

A) PEC were incubated with WR and A35Δ at an MOI of 10 for 10 h, fixed, permeabilized, and incubated with human polyclonal anti-VV hyperimmune sera. Viral antigens were measured by flow cytometry. B) BS-C-1 cells or PEC were infected with either WR or A35Δ mutant virus (MOI=10). Cells were collected at designated times post-infection and cell-associated virus was measured.