Figure 8. A35 decreases the amount of antigenic peptide associated with MHC class II.

Splenocytes from B10.A-H2^i5 H2-T18^a/(5R)SgSnJ mice (Jackson Labs) were infected for 3 h at an MOI=10, washed, and then incubated with no primary antibody or a biotin-conjugated anti-mouse Ea_56–68 peptide bound in the cleft of I-Ab Y-Ae followed by streptavidin-RPE. Samples were analyzed by flow cytometry, and MFI for Y-Ae were: Un 85, A35Δ 76, and WR 36. MFI for CLIP were Un 5.7, A35Δ 8.2, and WR 13.9.