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PEC were grown on coverslips for 4 h and then infected with WR or A35Δ virus at an MOI=10 for 2 hours. The cells were then fixed and permeabilized, and stained with a rabbit anti-A35 antibody and one of the following: goat anti-LAMP, goat anti-Rab7, goat anti-RhoB, goat anti-Rab7. The A35 protein was visualized with a FITC-conjugated secondary antibody, and the endosomal markers were visualized with Alexa Fluor 633.
