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. Author manuscript; available in PMC: 2011 Feb 1.
Published in final edited form as: Toxicon. 2009 Oct 21;55(2-3):619. doi: 10.1016/j.toxicon.2009.10.019

Fig. 2.

Fig. 2

DNA transfection reagent facilitates BoNT/A internalization of non-neuronal cell lines. Neuronal cell lines N2A, M17 and non-neuronal cell lines HEK293 (293), HIT-T15 were exposed to 10 nM BoNT/A for 24 hrs with (+) or without (−) pre-incubation with DNA transfection reagents, FuGene-HD or Lipofectamine 2000. Cell extracts were prepared and the extent of BoNT/A intoxication was measured by Western blotting to monitor SNAP25 cleavage.