Antibody titers of anti-Sm-p80 total IgG2a and IgG2b in immunized mice. Control prime boost (Cont PB; pcDNA3-ODN-2137), experimental prime boost (Exp PB; Sm-p80-pcDNA3-rSm-p80-ODN-10104), control protein vaccine, (Cont Prot Vacc; ODN-2137), and experimental protein vaccine (Exp Prot Vacc; rSm-p80-ODN-10104). a and c represent the prime boost experiments (IgG2a and IgG2b). The protein vaccination experiment is shown in b and d for IgG2a and IgG2b, respectively. The values represent the mean of three replicates ± standard error