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. Author manuscript; available in PMC: 2010 Nov 1.
Published in final edited form as: Anal Bioanal Chem. 2009 Sep 16;395(5):1453. doi: 10.1007/s00216-009-3109-x

Figure 1.

Figure 1

A representative 31P kinetic spectrum of myosin catalyzed ATP, obtained with 5 mM ATP at pH 7.0 and 21°C. The vertical axis shows timings for spectral acquisition. The peaks are assigned as: A (β-ATP); B (α-ATP and α-ADP); C (γ-ATP and β-ADP); D (Pi); E (AMP). The ATP hydrolysis can be characterized by decline of β-ATP. Subsequent ADP hydrolysis is also observable from the spectrum.