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. Author manuscript; available in PMC: 2011 Mar 1.
Published in final edited form as: Biomaterials. 2009 Dec 3;31(7):1626. doi: 10.1016/j.biomaterials.2009.11.035

Figure 8. Elastin in Arteries and Engineered Arterial Constructs.

Figure 8

Elastin production was quantified using an elastin-binding dye. (A) Soluble elastin concentrations in culture medium collected at the termination of culture of PGS or PLGA constructs were measured after centrifugation to remove any insoluble elastin (n = 4, 4, 10, and 10 from left to right). *PGS and PLGA constructs released significant amounts of soluble elastin into culture medium. (B) Insoluble elastin contents in porcine carotid arteries, PGS or PLGA constructs, and PGS or PLGA scaffolds were measured per unit wet weight after acid hydrolysis of each construct, which destroyed all proteins except elastin, and centrifugation to pellet insolubles (n = 4, 4, 4, 9, and 9 from left to right). *Arteries contained significantly more insoluble elastin than all other groups. ** PGS constructs contained significantly more insoluble elastin than PLGA constructs and uncultured scaffolds, but PLGA constructs did not contain significantly more insoluble elastin compared to uncultured scaffolds (even if the positive control was excluded). #The tunica adventitia of porcine carotid arteries was removed prior to testing.