Figure 7. Metabolic comparison of db₃₃₃/db₃₃₃, db/db and WT mice during a 24hr monitoring period.

A. Oxygen consumption in db₃₃₃/db₃₃₃, db/db and WT mice monitored for 24hrs. Both db/db and db₃₃₃/db₃₃₃ mice displayed similar oxygen consumption at all time points and these obese mutants showed significantly lower oxygen consumption than WT mice throughout both the light and dark cycle. B. CO₂ production in db₃₃₃/db₃₃₃, db/db and WT mice monitored for 24hrs. Both db/db and db₃₃₃/db₃₃₃ mice displayed similar levels of CO₂ production at all time points and these obese mutants showed significantly lower CO₂ production than WT mice throughout both the light and dark cycle. C. Respiratory Efficiency Ratio (RER) (VCO₂ /VO₂) in db₃₃₃/db₃₃₃, db/db and WT mice over a 24hr period. Both db₃₃₃/db₃₃₃ and db/db show a trend of lower RER relative to WT mice that is most pronounced in the dark cycle. db₃₃₃/db₃₃₃ mice show a consistently lower RER relative to db/db and WT mice. Data are plotted as mean ± SE for n = 4 animals of each genotype. Significant (P<0.05) differences between group means are denoted by asterics and were determined by one way ANOVA and post hoc Tukey test.