Skip to main content
. Author manuscript; available in PMC: 2011 Feb 5.
Published in final edited form as: Virology. 2009 Nov 28;397(1):23. doi: 10.1016/j.virol.2009.11.012

Figure 4.

Figure 4

HSV-2 Us3 kinase activity is disrupted in a D305A mutant. Equal volumes of cellular extracts prepared from Vero cells transfected with pCINeo vector (lane 1), a plasmid encoding WT HSV-2 Us3 (lane 2), or a plasmid encoding a D305A mutant of HSV-2 Us3 (lane 3) were analyzed by Western blotting. The upper panel was probed with Phospho-(Ser/Thr) PKA substrate antibody, the middle panel was probed with rat polyclonal HSV-2 Us3 antiserum and the loading control in the lower panel was probed with anti-actin monoclonal antibody. Molecular size markers indicated at the left of the panel are in kilodaltons.