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. Author manuscript; available in PMC: 2011 Feb 5.
Published in final edited form as: Virology. 2009 Nov 28;397(1):23. doi: 10.1016/j.virol.2009.11.012

Figure 8.

Figure 8

Predicted leucine-rich NESs in Us3. A. Predicted leucine-rich NESs in HSV-2 Us3. The top line depicts the most promiscuous consensus sequence encompassing 72% of experimentally verified leucine-rich NESs (la Cour et al., 2004); x’s correspond to any amino acid. Below are two tracts of sequence from HSV-2 Us3, designated 1 and 2, that fit this consensus sequence; amino acids corresponding to the x’s in the consensus sequence are shown in smaller font. Numbers in brackets below each predicted HSV-2 Us3 NES indicate the NES designation along with amino acid numbers that constitute the NES. B. Alignment of Us3 orthologues from other alphaherpesviruses. A portion of a multiple sequence alignment of Us3s from HSV-2, HSV-1, PRV, MDV, varicella zoster virus (VZV), bovine herpesvirus type 1 (BHV-1), and equine herpesvirus type 1 (EHV-1) generated using ClustalW v1.4 multiple sequence alignment software with a blosum similarity matrix is shown. Numbers in brackets correspond to the amino acid numbers of the portion of the protein shown. Identical amino acids are indicated with an asterisk; conserved amino acids are indicated with a period. Arrows above the alignment indicate the amino acids that constitute the two predicted leucine-rich NESs in HSV-2 Us3. The yes (Y) or no (N) designations at the far right indicate whether a sequence conforming to the consensus sequence shown in panel A is present in the portion of the Us3 protein shown.