FIG. 3.
Growth properties of the recombinant viruses. (A) Plaque sizes of UL11 mutants. Confluent monolayers of Vero cells were infected with the indicated viruses, and 4 days later, the cells were stained with crystal violet and imaged. Plaque size was determined by measuring 10 randomly selected plaques and represented as a percent relative to U1. (B–E) Single-step growth curve analyses of the mutants. Vero cells were infected with indicated virus and then acid-washed to inactivate any input virus that had not fused with the cellular membrane. At the indicated times, samples were collected, and virus titers were determined by plaque assay. The results of at least two independent experiments are shown for each mutant. (B) Comparison of the wild-type KOS strain and the recombinant carrying the bacterial artificial chromosome (“KOSBAC”). (C) Comparison of mutants that lack full-length UL11 expression (“Δ30–96”) or express UL11 from the UL35 locus (“U1”). (D and E) Analysis of recombinant viruses that encode UL11-derivatives with altered acylation signals. Solid lines denote PFU associated with the cells, whereas dashed lines represent PFU released into the medium.