Table 1.
Primer sequences for KIR analysis using MALDI-TOF.
| Assay Name | Forward Capture Primer | Reverse Capture Primer | hME Primer |
|---|---|---|---|
| 2DL1.2DS1.D1.S.tri | ACGTTGGATGAAGGCCAACTTCTCCATCA | ACGTTGGATGGTGAGTAACAGAACCGTAGC | GGTCCCTGCCAGGTCTTGC |
| 2DL1.D1.Gb | ACGTTGGATGACTTCTCCATCAGTCGCATGAC | ACGTTGGATGATGATCACGATGTCCAGAGG | GTCACTGGGAGCTGACA |
| 2DL1.D2.G.no004 | ACGTTGGATGCAGGGCCCAAGGTCAACG | ACGTTGGATGGACTTTGACCACTCGTAT | ATGCTTCGGCTCTTTCC |
| 2DL1.no005.2DL2.004.TC.S | ACGTTGGATGGTAATGGACCAAGAGTCTGC | ACGTTGGATGCGGGCCGAGGAGTACCTACCT | CGCTATTCGCTGTTCTGTT |
| 2DL2.001.2.3.D1.G | ACGTTGGATGCCTGCAATGTTGGTCAGATG | ACGTTGGATGGGAGCTGACAACTGATAGGG | CATGATGGGGTCTCCAA |
| 2DL2.004.TC.G | ACGTTGGATGGTAATGGACCAAGAGTCTGC | ACGTTGGATGGGCCGAGGAGTACCTACCT | GAAACAGAACAGCGAATA |
| 2DL2.D2.S | ACGTTGGATGGAGCTCCTATGACATGTACC | ACGTTGGATGGCCTGGAATGTTCCGTTGACCTTG | CCCTGCAGAGAACCTAC |
| 2DL3.2DL2.D1.S | ACGTTGGATGGAGTCCACAGAAAACCTTCCCTCC | ACGTTGGATGAGTGTCCTTAAACTTCCCTTCTC | CTTCTGATTTCACCAGG |
| 2DL3.TC.S.INT | ACGTTGGATGGTAACCCCAGACACCTGCATG | ACGTTGGATGCTGCTTCGTGAGACTTACTT | TCTCCTTCATCGCTGGTGCT |
| 2DL4.DO.G | ACGTTGGATGCCCTGAGCTCTACAACAGAA | ACGTTGGATGTGCCGACCACTCAGTGGG | TGGAACAGTTTCCTCAT |
| 2DL4.TC.G | ACGTTGGATGAGGTGACATACGCACAGTTG | ACGTTGGATGATCTGTTGAGGGTCTCTTGC | CACAGTTGGATCACTGC |
| 2DL5.D2.G | ACGTTGGATGGACTTTCCTCTGGGCCCTG | ACGTTGGATGTGACAGAAACAAGCAGTGGG | CCACGGAGGGACCTACA |
| 2DL5.TC.G | ACGTTGGATGCAAGACCCTCAGGAGGTGAC | ACGTTGGATGCTTGGGCCTCTGAGAAGGG | CACTGCGTTTTCACACAGA |
| 2DL5sub1 | ACGTTGGATGAGGACAAGCCCTTGCTGTCT | ACGTTGGATGCAAGACGAGAGCGACACA | GTCCTCCTCGAGGCACCACAG |
| 2DL5sub2 | ACGTTGGATGCCCTGAGCTCTACAACAA | ACGTTGGATGGACATGAGTCCTCTGACCTG | CGCTCCCCCATTGAGTGGTC |
| 2DL5sub3 | ACGTTGGATGCCCTGAGCTCTACAACAA | ACGTTGGATGGACATGAGTCCTCTGACCTG | GCAACCCCCTGGTGATC |
| 2DL5sub4 | ACGTTGGATGCCACGGAGGGACCTACAC | ACGTTGGATGGTGACAGAAACAAGCAGTGG | GTGAGTCATGGAGAGAGC |
| 2DL5sub5 | ACGTTGGATGGATCTTGGCTTAGCATTTGG | ACGTTGGATGCTGCGTTTTCACACAGAC | CTTCTCAGAGGCCCAAG |
| 2DL5sub6 | ACGTTGGATGTAAGGTGGCGCCTCCTTCTC | ACGTTGGATGCAAGACGAGAGCGACACA | AGCAAGGGCTTGTCCTG |
| 2DP1.D0.G | ACGTTGGATGGGGTTTAACAACTTCAGTCTGT | ACGTTGGATGTGTGCTGGGGTCACAGGGCC | ATTCTGTTGTAGAGCTCAG |
| 2DS1.D2.G | ACGTTGGATGATTCCAGGCCAACTTTCCTC | ACGTTGGATGATGGAGAGTCACGGAAAGAG | CATCTGTAGGTCCCTCC |
| 2DS2.D1.G | ACGTTGGATGCCTGCAATGTTGGTCAGATG | ACGTTGGATGAGAAGTTGGCCTTGGAGACC | GCACAGAGAGGGGAAGT |
| 2DS2.D2.G | ACGTTGGATGGTCTATATGAGAAACCTTC | ACGTTGGATGGGACAAGGTCACGCTCTCTC | CACGCTCTCTCCTGCCA |
| 2DS3.D1.G | ACGTTGGATGAAGGCCAACTTCTCCATCGG | ACGTTGGATGCTGTGATCACGATGTCCAG | CACTCCCCCTATCAGTT |
| 2DS3.D2.S | ACGTTGGATGAGGTCAACGGAACATTCCAGGCCG | ACGTTGGATGAAGAGCCGAAGCATCTGTAG | CATCTGTAGGTTCCTCC |
| 2DS4.D1.G | ACGTTGGATGAGAGACAGTCATCCTGCAATG | ACGTTGGATGATGGAGAAGTTGGCCTTGGA | GAAGTGCTCAAACATGACATC |
| 2DS4.D2.S | ACGTTGGATGGCATCAACGGAACATTCCAGGCC | ACGTTGGATGGAGCTCTGTGACGGAAACAA | TCGGCTCTTTCCGTGAC |
| 2DS4del.sub | ACGTTGGATGCGGTTCAGGCAGGAGAGAAT | ACGTTGGATGTTGACCACTCGTAGGGAGC | CCTTGTCCTGCAGCTCC |
| 2DS5.D1.G | ACGTTGGATGACACTTTGCGCCTCATTGGAG | ACGTTGGATGGTGAGTAACAGAACCGTAG | GACCGATGGAGAAGTTG |
| 2DS5.D2.G | ACGTTGGATGAGGCCCATGAACGTAGGCTCC | ACGTTGGATGAAGAGCCGAAGCATCTGTAG | CTCCGTGGGTGGCAGGG |
| 3DL1.TC.S | ACGTTGGATGCACTGCGTTTTCACACAGAG | ACGTTGGATGATGGGCAGGAGACAACTTTG | AGGCCCAAGACACCCCC |
| 3DL2.D1.G | ACGTTGGATGAAGGCCAACTTCTCCATCGG | ACGTTGGATGTGGGAGCTGACAACTGATAG | GTGAGGAACAGAACCATA |
| 3DL2.TC.G | ACGTTGGATGGATGAACAAGACCCTCAGGAGGTG | ACGTTGGATGTACACGCTGGTATCTGTT | GCCTCTGAGAAGGGCGA |
| 3DL3.D1.G | ACGTTGGATGGATCACTGAGGACCCCTTGC | ACGTTGGATGTCATGGGACCCATGGAATAG | AATAGTTGACCTGGGAACCC |
| 3DL3.D2.G | ACGTTGGATGAGAATGTGACCTTGTCCTGC | ACGTTGGATGCAGTGAGCCTAAGTTCACCG | GGATAGATGGTAAATGTCAAA |
| 3DP1.D2.G2 | ACGTTGGATGTGGGAAACCTTCTCTCTCAGCC | ACGTTGGATGGAGCTGCAGGACAAGGTCAC | CTCTCTCAGCCCAGCCG |
| 3DS1.3DL1.D1.S | ACGTTGGATGCAAGGCCAATTTCTCCATCG | ACGTTGGATGGGGAGCTGACAACTGATAGG | CTGTAGGTCCCTGCAAGGGCA |
| 3DS1.DO.S | ACGTTGGATGTGTGTAGTTCCCTGCATGTG | ACGTTGGATGTCATGCTATACAAAGAAGAC | AGGGCTCATGTTGAAGC |
| 3DS1.TC.S.INT | ACGTTGGATGAACTGCTATGATTAGCTTC | ACGTTGGATGGATGAAGGAGAAAGAAGAGGAGGA | GAATGTGCAGGTGTCTG |