Skip to main content
. Author manuscript; available in PMC: 2010 Oct 1.
Published in final edited form as: Immunogenetics. 2009 Sep 30;61(10):663. doi: 10.1007/s00251-009-0396-5

Table 2.

Primer sequences for HLA-B (Bw4 ligand, Bw6) and HLA-C (C1, C2) ligand group analysis using MALDI-TOF.

Primer Sequence Region
HLACP1 ACG TTG GAT GGG AGG GAA DCG GCC TCT GSG GA PCR HLA-C upstream primer
HLACP2 ACG TTG GAT GGC CCC RGG CCG GGG TCA CTC AC PCR HLA-C, HLA-B downstream primer
HLAPBforw ACG TTG GAT GGG GAG GAG MGA GGG GAC CGC AG PCR HLA-B upstream primer
hlaC80 GGT TGT AGT AGC CGC GCA G Extension primer HLA-C aa80
hlaC77 ACA GGC TGA CCG AGT GA Extension primer HLA-C aa77
hlaB83hME GCT CTG GTT GTA GTA GC Extension primer HLA-B aa83

Notes: The first 10 bp of the PCR primers are primer tags (ACGTTGGATG), specified by the SEQUENOM™ AssayDesigner. D = A, G, or T; S = G or C; R =A or G; M = A or C