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Mice were prepared with a commercial depilatory agent, 6mm punch biopsies made and a silicone stent fixed around the wound with Krazy Glue, and 4.0 prolene suture as described by (Gurtner). A Telfa pledget was then placed in the wound bed and tegaderm is glued to the silicone sheeting and the surrounding skin, creating a pocket into which we injected MMP-9 (right sided wound) or vehicle (left sided wound). Dressing was reinforced with Koban.
