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. Author manuscript; available in PMC: 2011 Mar 1.
Published in final edited form as: Biomaterials. 2009 Nov 14;31(7):1944. doi: 10.1016/j.biomaterials.2009.10.060

Fig. 4.

Fig. 4

ELISA studies analyzing GPRP-FnIII9-10 affinity for fibrinogen. (A) Affinity assays demonstrating binding between immobilized GPRP-FnIII9-10-C, but not GSPE-FnIII9-10-C, and fibrinogen. ELISA plate readouts (Abs450nm) were normalized against the maximum signal obtained using GPRPFPAC peptide (Genscript) at a coating concentration of 20 μg/mL. (B) Specificity assays demonstrating that GPRP and GPRV peptides compete with immobilized GPRP-FnIII9-10 for fibrinogen. ELISA plate readouts were normalized against the signals obtained in the presence of the non-binding GSPE peptide to correct for osmolarity.