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. Author manuscript; available in PMC: 2011 Feb 5.
Published in final edited form as: Virology. 2009 Nov 24;397(1):155. doi: 10.1016/j.virol.2009.11.002

Figure 2. Interaction between PIV5 M protein and AmotL1 detected with a pairwise yeast two-hybrid assay.

Figure 2

Yeast cells (strain L40) were transformed with plasmids corresponding to the indicated baits, and then transformed a second time with plasmids corresponding to the indicated preys. Growing yeast colonies were selected, suspended in water, and spotted onto a nitrocellulose membrane in replicates of four. Yeast cells on the membrane were disrupted with freeze-thaw cycles, and beta-galactosidase activity was detected using the colorimetric substrate X-gal.