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. 2008 Sep 11;189(6):410–419. doi: 10.1159/000155225

Fig. 4.

Fig. 4.

Smooth muscle maintains smooth muscle actin expression in culture for 7 days, but is attenuated by FGF-2. A Sagittal section of E14.5 esophagus after 7 days of culture shows robust expression of smooth muscle actin in all 3 smooth muscle layers of the esophagus (a = muscularis mucosa, b = muscularis externa inner layer, c = muscularis externa outer layer). B Smooth muscle actin expression is attenuated after 7 days of culture when FGF-2 is added to the culture media (a muscularis mucosa, b muscularis externa inner layer, c muscularis externa outer layer). Note the loss of actin in the outer gastric muscle layers. C Myogenin expression remains unaffected by culture in FGF-2. Scale bars = 500 μm. Inset PD98059 inhibits ERK1/2 phosphorylation by FGF-2. E14.5 esophageal lysates, immunoblotted with anti-phospho-ERK1/2 or anti-pan-ERK antibodies, show complete inhibition of ERK1/2 phosphorylation by FGF-2 after concurrent treatment with MEK inhibitor PD98059 (upper panel, lanes 3 and 4). Esophagi treated with FGF-2 alone show robust phosphorylation of ERK1 and ERK2 (upper panel, lanes 1 and 2). Total ERK expression is similar for all samples (lower panel).