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. 1993 Apr;61(4):1571–1575. doi: 10.1128/iai.61.4.1571-1575.1993

Localization of the virulence-associated genes pilA, pilR, rpoN, fliA, fliC, ent, and fbp on the physical map of Pseudomonas aeruginosa PAO1 by pulsed-field electrophoresis.

M A Farinha 1, S L Ronald 1, A M Kropinski 1, W Paranchych 1
PMCID: PMC281404  PMID: 8454366

Abstract

Seven virulence-associated genes have been placed on a genomic map of Pseudomonas aeruginosa PAO1, using pulsed-field electrophoresis, on the basis of the previous physical maps of Romling et al. (U. Romling, M. Duchene, D. Essar, D. Galloway, C. Guidi-Rontani, D. Hill, A. Lazdunski, R. Miller, K. Schleifer, D. Smith, H. Toschka, and B. Tummler, J. Bacteriol. 174:327-330, 1992; U. Romling, D. Grothues, W. Bautsch, and B. Tummler, EMBO J. 8:4081-4089, 1989) and Ratnaningsih et al. (E. Ratnaningsih, S. Dharmsthiti, V. Krishnapillai, A. Morgan, M. Sinclair, and B. W. Holloway, J. Gen. Microbiol. 136:2351-2357, 1990). The new locations for the outer membrane enterobactin iron-siderophore receptor ent gene (41 to 42 min) and the fliA gene (59 to 61 min), which encodes a minor sigma factor of RNA polymerase, are given. The pilA (the pilin structural gene), pilR (a pilin regulatory gene), and rpoN (encoding another minor sigma factor of RNA polymerase) genes map together at 71 to 75 min, locations correcting the previously reported values (V. Shortridge, M. Pato, A. Vasil, and M. Vasil, Infect. Immun. 59:3596-3603, 1990). The fbp gene (28 to 29 min), which encodes an outer membrane ferripyochelin-binding protein of low molecular weight, and the fliC gene (64 to 66 min), the flagellin structural gene, were determined to lie in the previously reported locations.

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Selected References

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  1. Ankenbauer R. G. Cloning of the outer membrane high-affinity Fe(III)-pyochelin receptor of Pseudomonas aeruginosa. J Bacteriol. 1992 Jul;174(13):4401–4409. doi: 10.1128/jb.174.13.4401-4409.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Cox C. D. Iron reductases from Pseudomonas aeruginosa. J Bacteriol. 1980 Jan;141(1):199–204. doi: 10.1128/jb.141.1.199-204.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Cox C. D. Iron transport and serum resistance in Pseudomonas aeruginosa. Antibiot Chemother (1971) 1985;36:1–12. doi: 10.1159/000410466. [DOI] [PubMed] [Google Scholar]
  4. Inouye S., Yamada M., Nakazawa A., Nakazawa T. Cloning and sequence analysis of the ntrA (rpoN) gene of Pseudomonas putida. Gene. 1989 Dec 21;85(1):145–152. doi: 10.1016/0378-1119(89)90474-5. [DOI] [PubMed] [Google Scholar]
  5. Irvin R. T., Doig P., Lee K. K., Sastry P. A., Paranchych W., Todd T., Hodges R. S. Characterization of the Pseudomonas aeruginosa pilus adhesin: confirmation that the pilin structural protein subunit contains a human epithelial cell-binding domain. Infect Immun. 1989 Dec;57(12):3720–3726. doi: 10.1128/iai.57.12.3720-3726.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Ishimoto K. S., Lory S. Formation of pilin in Pseudomonas aeruginosa requires the alternative sigma factor (RpoN) of RNA polymerase. Proc Natl Acad Sci U S A. 1989 Mar;86(6):1954–1957. doi: 10.1073/pnas.86.6.1954. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Ishimoto K. S., Lory S. Identification of pilR, which encodes a transcriptional activator of the Pseudomonas aeruginosa pilin gene. J Bacteriol. 1992 Jun;174(11):3514–3521. doi: 10.1128/jb.174.11.3514-3521.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Kustu S., Santero E., Keener J., Popham D., Weiss D. Expression of sigma 54 (ntrA)-dependent genes is probably united by a common mechanism. Microbiol Rev. 1989 Sep;53(3):367–376. doi: 10.1128/mr.53.3.367-376.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Montie T. C., Drake D., Sellin H., Slater O., Edmonds S. Motility, virulence, and protection with a flagella vaccine against Pseudomonas aeruginosa infection. Antibiot Chemother (1971) 1987;39:233–248. doi: 10.1159/000414349. [DOI] [PubMed] [Google Scholar]
  10. O'Hoy K., Krishnapillai V. Recalibration of the Pseudomonas aeruginosa strain PAO chromosome map in time units using high-frequency-of-recombination donors. Genetics. 1987 Apr;115(4):611–618. doi: 10.1093/genetics/115.4.611. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Ohnishi K., Kutsukake K., Suzuki H., Iino T. Gene fliA encodes an alternative sigma factor specific for flagellar operons in Salmonella typhimurium. Mol Gen Genet. 1990 Apr;221(2):139–147. doi: 10.1007/BF00261713. [DOI] [PubMed] [Google Scholar]
  12. Pasloske B. L., Finlay B. B., Paranchych W. Cloning and sequencing of the Pseudomonas aeruginosa PAK pilin gene. FEBS Lett. 1985 Apr 22;183(2):408–412. doi: 10.1016/0014-5793(85)80821-8. [DOI] [PubMed] [Google Scholar]
  13. Poole K., Young L., Neshat S. Enterobactin-mediated iron transport in Pseudomonas aeruginosa. J Bacteriol. 1990 Dec;172(12):6991–6996. doi: 10.1128/jb.172.12.6991-6996.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Ratnaningsih E., Dharmsthiti S., Krishnapillai V., Morgan A., Sinclair M., Holloway B. W. A combined physical and genetic map of Pseudomonas aeruginosa PAO. J Gen Microbiol. 1990 Dec;136(12):2351–2357. doi: 10.1099/00221287-136-12-2351. [DOI] [PubMed] [Google Scholar]
  15. Römling U., Duchéne M., Essar D. W., Galloway D., Guidi-Rontani C., Hill D., Lazdunski A., Miller R. V., Schleifer K. H., Smith D. W. Localization of alg, opr, phn, pho, 4.5S RNA, 6S RNA, tox, trp, and xcp genes, rrn operons, and the chromosomal origin on the physical genome map of Pseudomonas aeruginosa PAO. J Bacteriol. 1992 Jan;174(1):327–330. doi: 10.1128/jb.174.1.327-330.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Römling U., Grothues D., Bautsch W., Tümmler B. A physical genome map of Pseudomonas aeruginosa PAO. EMBO J. 1989 Dec 20;8(13):4081–4089. doi: 10.1002/j.1460-2075.1989.tb08592.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Shortridge V. D., Pato M. L., Vasil A. I., Vasil M. L. Physical mapping of virulence-associated genes in Pseudomonas aeruginosa by transverse alternating-field electrophoresis. Infect Immun. 1991 Oct;59(10):3596–3603. doi: 10.1128/iai.59.10.3596-3603.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Sokol P. A. Surface expression of ferripyochelin-binding protein is required for virulence of Pseudomonas aeruginosa. Infect Immun. 1987 Sep;55(9):2021–2025. doi: 10.1128/iai.55.9.2021-2025.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Starnbach M. N., Lory S. The fliA (rpoF) gene of Pseudomonas aeruginosa encodes an alternative sigma factor required for flagellin synthesis. Mol Microbiol. 1992 Feb;6(4):459–469. doi: 10.1111/j.1365-2958.1992.tb01490.x. [DOI] [PubMed] [Google Scholar]
  20. Totten P. A., Lara J. C., Lory S. The rpoN gene product of Pseudomonas aeruginosa is required for expression of diverse genes, including the flagellin gene. J Bacteriol. 1990 Jan;172(1):389–396. doi: 10.1128/jb.172.1.389-396.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Wallace R. B., Miyada C. G. Oligonucleotide probes for the screening of recombinant DNA libraries. Methods Enzymol. 1987;152:432–442. doi: 10.1016/0076-6879(87)52050-x. [DOI] [PubMed] [Google Scholar]
  22. West S. E., Iglewski B. H. Codon usage in Pseudomonas aeruginosa. Nucleic Acids Res. 1988 Oct 11;16(19):9323–9335. doi: 10.1093/nar/16.19.9323. [DOI] [PMC free article] [PubMed] [Google Scholar]

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