Figure 3.

Real time RT-PCR. HMEC cultures were exposed to vector alone (VC) or MMP-9-PEX SNB19 (PX1,PX2) cell conditioned medium for 24 h. Conditioned media were removed, cells were lysed, and total RNA extracted with Trizol Reagent. Reverse transcription reactions were carried out on total RNA with the cDNA cycle kit using the random primers. Gene transcripts for MMP-2 (a), MMP-9 (b), VEGF (c) and VEGFR2 (d) were measured using real-time reverse transcription-PCR. Transcript of β-actin was used as the internal control. Data represent a result of triplicate samples from two independent experiments. **p< 0.01. ELISA. HMEC cultures were exposed to vector or MMP-9-PEX SNB19 cell conditioned medium. Culture supernatants and lysates were subjected to the measurements for VEGF (e) and VEGFR2 (f) by ELISA respectively. Data represent a result of triplicate determinations from two independent experiments. **p< 0.01