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. Author manuscript; available in PMC: 2010 Jan 30.
Published in final edited form as: Dev Biol. 2008 Sep 3;324(1):1. doi: 10.1016/j.ydbio.2008.08.020

Fig. 3. Semaphorin expression during facial visceromotor axon guidance.

Fig. 3

(A-C) Wholemount visualisation of the GSPN nerve (VIIgspn) by neurofilament immunofluorescence (A,C) or anterograde DiI labelling (B) at 10.5 (A), 11.0 (B) or 12.5 dpc (C); ventral view, anterior up. The lower jaw including their trigeminal nerve branches was removed to afford better visualisation of the VIIgspn. At 10.5 dpc, VIIgspn axons extended from the main nerve trunk (arrows in A) towards Rathke's pouch (circled in A), but these axons had retracted by 11.0 dpc (arrows in B) and 12.5 dpc (arrows in C). (D,E) Wholemount in situ hybridisation at 10.5 dpc for Sema3a (D) and Sema3f (E) and at 12.5 dpc for the sphenopalatine ganglion (SPg) marker Phox2b (F). The VIIgspn encounters two areas of high semaphorin expression as it extends towards its target site, one in the second pharyngeal arch (arrows in D,E) and one surrounding Rathke's pouch (arrowheads in D,E). At 12.5 dpc, VIIgspn axons arborated in the area where the SPg had formed (compare circled areas in C with F). Scale bars: (A,B,D,E) and (C,F) 500 μm.