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. 2009 Jul 16;517(3):296–312. doi: 10.1002/cne.22161

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Copyright © 2009 Wiley-Liss, Inc., A Wiley Company

Re-use of this article is permitted in accordance with the Creative Commons Deed, Attribution 2.5, which does not permit commercial exploitation.

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Multiple forms of PIP4Kγ are differentially expressed in brain regions. A: PIP4Kγ was detected as three different bands (47, 48, and 49 kDa) by 8% SDS-PAGE and Western blotting. The 49-kDa band was differentially expressed in cerebral cortex, hippocampus, spinal cord, cerebellum, and brainstem and was not present in the olfactory bulb. B: Only the 47-kDa band remained after lysates were pretreated with calf intestinal alkaline phosphatase (CIAP) and Western blotted with anti-PIP4Kγ antibody. Results are representative of at least three experiments.