Figure 12.

Subcellular localization of PIP4Kγ in neurons. Endogenous PIP4Kγ was detected in the cell body and dendritic trees of cerebellar Purkinje cells (A), olfactory bulb mitral cells (B), and large neurons in dorsal root ganglion (drg) preparations (C). PIP4Kγ was mostly excluded from the nucleus and was present in a vesicular compartment in the cytoplasm. This compartment seemed to have a stronger, but partial, colocalization with the cis-Golgi structural marker GM130 (J–L) than with the ER resident calnexin (D–F) or the -ergic marker p115 (G–I), although this could not be fully characterized in brain tissue. Images are representative of results from at least three stained sections. Scale bars = 20 μm in A–C; 20 μm in F (applies to D–F); 20 μm in I (applies to G–I); 20 μm in L (applies to J–L).