Fig. 2.

Growth inhibitory effect of PAF on the A. nidulans mutants ΔmpkA and alcA-PkcA compared with the recipient strains GR5 and R153 respectively. A total of 2 × 10³ conidia were point inoculated on agar plates (CM for GR5 and ΔmpkA, repressive MM containing 1% glucose according to Ronen et al., 2007; for R153 and alcA-PkcA) containing the appropriate supplements and 0–100 μg ml⁻¹ PAF. The plates were incubated at 37°C for 48 h (GR5 and ΔmpkA) or 72 h (R153 and alcA-PkcA mutant).