Table 2.
Colocalization patterns of Yfp-MinD and wild-type and mutant MinE-Cfp.
| Localization pattern |
MinE |
|||||
|---|---|---|---|---|---|---|
| MinD polar zone | MinE ring | WT | R10G/ K11E/ K12E | K19E/ R21G | R29G/ R30G/ R31G | D45A/ V49A |
| ≤ 0.5 cell length | + | 55.0% | 8.7% | 0.0% | 64.2% | 0.3% |
| ≤ 0.5 cell length | − | 5.6% | 9.9% | 0.0% | 1.8% | 50.3% |
| ≥ 0.5 cell length | + | 8.3% | 11.1% | 0.0% | 1.8% | 0.0% |
| ≥ 0.5 cell length | − | 0.6% | 35.1% | 0.0% | 0.0% | 32.9% |
| Off cell pole | +/− | 3.9% | 0.7% | 0.0% | 4.2% | 0.3% |
| Double polar zone | +/− | 0.0% | 3.5% | 0.0% | 0.0% | 2.0% |
| Peripherala | Disperseda/focusa | 20.6% | 28.2% | 100.0% | 27.9% | 6.9% |
| Not clear | Not clear | 6.1% | 2.8% | 0.0% | 0.0% | 7.4% |
| n | 180 | 425 | 506 | 159 | 392 | |
a
Please see Fig. 5A for peripheral (p), dispersed (d) and focus (f) patterns of localization.