Fig. 3.

Subcellular localization of TaSnRK2.4 in onion epidermal cells. Cells were bombarded with constructs carrying GFP or TaSnRK2.4–GFP as described in the Materials and methods. GFP and TaSnRK2.4–GFP fusion proteins were transiently expressed under control of the CaMV 35S promoter in onion epidermal cells and observed with a laser scanning confocal microscope. Images were taken in the dark field for green fluorescence (1, 4), while the outline of the cell (2, 5) and the combination (3, 6) were photographed in a bright field. (This figure is available in colour at JXB online.)