Fig. 3.

PRINS analysis performed on metaphase chromosomes of P. coccineus. The reactions were performed with PcPCNA1 specific primers (A) and with PcPCNA-like1 specific primers (B) in the presence of DIG-12-dUTP and 3 units of Taq polymerase. After the reaction, slides were incubated with anti-DIG-fluorescein antibody and preparations were examined with the OLYMPUS BX60 Research System Microscope. The observed signals are denoted by white arrows. Each pictures shows a single diploid cell (2n=22).