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. 2009 Dec 10;61(3):873–888. doi: 10.1093/jxb/erp354

Fig. 7.

Fig. 7.

Analysis of PcPCNA1 and PcPCNA-like1 binding to p21 peptide. Biotinylated p21 peptide was attached to streptavidin–agarose beads and incubated with 1 μg of the recombinant protein followed by extensive washing. The samples were then separated in 12% SDS-PAGE and stained with Coommassie dye. Lane 1: molecular mass marker; lane 2: streptavidin–agarose beads without p21, reacted with PcPCNA1; lane 3: beads with p21, reacted with PcPCNA1; lane 4: beads without p21, reacted with PcPCNA-like1; lane 5: beads with p21 reacted with PcPCNA-like1; lane 6: beads without p21, reacted with HsPCNA; lane 7: beads with p21, reacted with HsPCNA.