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. Author manuscript; available in PMC: 2010 Dec 1.
Published in final edited form as: Ann Surg. 2009 Dec;250(6):995. doi: 10.1097/SLA.0b013e3181b0ebf8

FIGURE 3.

FIGURE 3

Severity of hypoxia of Kupffer cells following trauma-hemorrhage or hypoxia. Pimonidazole was injected intravenously before trauma-hemorrhage or hypoxic air inhalation at a dosage of 60 mg/Kg. Upon harvesting, Kupffer cells were prepared as described in Materials and Methods and stained with APC-conjugated anti-F4/80 antibodies and FITC-conjugated antipimonidazole antibodies. Data are mean ± SE; n = 6 animals/group. #P < 0.05 compared with sham.