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. Author manuscript; available in PMC: 2010 Dec 1.
Published in final edited form as: Ann Surg. 2009 Dec;250(6):995. doi: 10.1097/SLA.0b013e3181b0ebf8

FIGURE 5.

FIGURE 5

Expression of activated HIF-1α in Kupffer cells following trauma-hemorrhage or hypoxia. Male C3H/HeN mice were subjected to sham treatment, trauma-hemorrhage or hypoxia as described in Materials and Methods. Each group of animals was given Wortmannin (1 mg/Kg body weight, BW), YC-1 (5 mg/Kg BW) or vehicle at maximum bleedout time or 30 minutes after induction of hypoxia. Upon harvesting, Kupffer cells were isolated, nuclear proteins were extracted, and HIF-1α levels were determined as described in Materials and Methods. Data are mean ± SE; n = 6 animals/group. #P < 0.05 compared with all the other groups.