Figure 1.

Tropomyosins used for reconstitution. The structure of E. coli-synthesized Tm used for our study, highlighting the 7 quasirepeat regions. The N-terminal amino acid residue for each region is shown underneath nfTm. Yellow color represents the region(s) deleted in the respective mutants. ∆23Tm was previously studied [13, 14] and is included here for the sake of comparison. nfTm is the full-length protein and was used as a control. The N-terminus of ∆23Tm has the Ala-Ser substitution, whereas the N-termini of ∆2Tm, ∆3Tm, and nfTm do not have this substitution.